The Impact of Fire Severity on Soil Microbial Biomass and Community Composition in Larix gmelinii Forests

doi:10.13466/j.cnki.lczyyj.2024.06.005

Abstract

Abstract:

Understanding the response of soil microbial communities to fire severity in flat and sloping forests,as well as the factors influencing microbial composition in fire slash,is crucial for guiding ecological restoration efforts.The fire slash of Larix gmelinii forest after restoration for 1 year from low,medium and high severity fires in the Bilahe Forestry Bureau of Inner Mongolia were selected as research subjects.Using phospholipid fatty acid(PLFA)analysis,soil microbial biomass and community composition were assessed at soil depth from 0 to 5 cm.Differences in soil microbial biomass,community composition and diversity in flat and sloping lands of different fire severities were analyzed.The combined effects of fire severity,slope,and soil nutrient indicators on soil microbial biomass were further explored by redundancy analysis(RDA)and structural equation modeling(SEM).1)Among microbial groups in both control plots and burnt areas,Gram-negative bacteria had the highest biomass,followed by Bacteria and Grampositive bacteria,then Fungi and Actinomycetes,and the smallest was Arbuscularmycorrhizal fungi.2)The biomass of Arbuscularmycorrhizal fungi was hardly affected by fire.Except for the Arbuscularmycorrhizal fungi,the biomass of microbial groups and community diversity indices of flat soil increased but were insignificant after fire,while in sloping soils,microbial biomass increased significantly(P<0.05).Biomass and community diversity indices of soil microbial groupsinsloping soil were largest after low severity fires.The change of Grampositive bacterial biomass was the main driving force of the response of soil microbial community to fire severities in sloping land.3)Fire severity,slope,and soil nutrient indicators all significantly(P<0.01)affected the biomass of microbial groups,with the direct effect in the order of soil nutrient indicators,fire severity,and slope.Soil nutrient indicators of water-soluble organic carbon content were strongly and positively correlated with the biomass of fungi,and ammonium nitrogen content was positively correlated with the biomass of Gram-positive bacteria.Consequently,fire severity and slope not only have a direct effect on soil microbial biomass and community composition,but also have an indirect effect on the biomass of soil microbial groups by regulating soil nutrient indicators.

Key words: fire severity, Larix gmelinii, sloping land, soil microbes, PLFA

CLC Number:

S762

YANG Guang, WANG Xinyu, MA Yunjia, LIU Zhaoyan, WANG Lixuan, SUN Jian. The Impact of Fire Severity on Soil Microbial Biomass and Community Composition in Larix gmelinii Forests[J]. Forest and Grassland Resources Research, 2024, (6): 35-44.

Figures/Tables 8

Tab.1

Fig.1

Fig.2

Tab.2

Tab.3

Fig.3

Tab.4

Soil nutrient characteristics variables and corresponding RDA analysisresults

土壤养分特征	解释度/%	贡献度/%	伪F值	P
WSOC	8.3	16.9	3.0	0.034
WSN	5.0	10.0	1.8	0.130
$N H 4 +$ -N	7.0	14.1	2.7	0.046
TN	3.9	7.9	1.5	0.184
TOC	3.8	7.8	1.5	0.200
MBN	5.3	10.7	2.2	0.080
MBC	7.9	16.1	3.7	0.026

Tab.4

Fig.4

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微生物类群	特征脂肪酸
真菌	18:1 ω9c;18:2 ω6c;18:3 ω6c
细菌	14:0;15:0;16:0;17:0;18:0
丛枝菌根真菌	16:1 ω5c
放线菌	16:0 10-methyl、17:0 10-methyl、18:0 10-methyl
革兰氏阳性菌	13:0 iso、13:0 anteiso、14:0 iso、15:0 iso、15:0 anteiso、16:0 iso、17:0 iso、17:0 anteiso、18:0 iso
革兰氏阴性菌	14:1 ω5c、15:1 ω6c、16:1 ω7c、16:1 ω7c alcohol、16:1 ω9c、18:1 ω5c、18:1 ω7c、22:1 ω9c

低烈度			中烈度			高烈度
特征脂肪酸	VIP值	微生物种类	特征脂肪酸	VIP值	微生物种类	特征脂肪酸	VIP值	微生物种类
18:1 ω7c	1.689	革兰氏阴性菌	18:1 ω7c	1.729	革兰氏阴性菌	18:1 ω7c	1.369	革兰氏阴性菌
16:0 iso	1.580	革兰氏阳性菌	18:0 iso	1.612	革兰氏阳性菌	18:2 ω6c	1.312	真菌
14:0 iso	1.501	革兰氏阳性菌	15:0 anteiso	1.590	革兰氏阳性菌	15:0	1.308	细菌
15:0 anteiso	1.389	革兰氏阳性菌	18:0 10-methyl	1.514	放线菌	16:1 ω5c	1.305	丛枝菌根真菌
18:0 iso	1.386	革兰氏阳性菌	15:0 iso	1.410	革兰氏阳性菌	18:3 ω6c	1.273	真菌
17:0 anteiso	1.315	革兰氏阳性菌	14:0	1.390	细菌	14:0 iso	1.269	革兰氏阳性菌
15:0 iso	1.313	革兰氏阳性菌	16:0 iso	1.270	革兰氏阳性菌	18:0 iso	1.268	革兰氏阳性菌
18:2 ω6c	1.232	真菌	18:1 ω5c	1.206	革兰氏阴性菌	13:0 anteiso	1.233	革兰氏阳性菌
14:0	1.195	细菌	18:1 ω9c	1.194	真菌	14:0	1.219	细菌
18:1 ω5c	1.188	革兰氏阴性菌	14:0 iso	1.107	革兰氏阳性菌	18:1 ω9c	1.177	真菌
16:1 ω5c	1.155	丛枝菌根真菌	15:0	1.102	细菌	15:0 anteiso	1.169	革兰氏阳性菌
17:0 iso	1.148	革兰氏阳性菌	17:0 iso	1.070	革兰氏阳性菌	15:0 iso	1.146	革兰氏阳性菌
18:0	1.033	放线菌细菌	13:0 iso	1.056	革兰氏阳性菌	18:0 10-methyl	1.110	放线菌
			18:2 ω6c	1.044	真菌	17:0 iso	1.019	革兰氏阳性菌

坡地类型	火烈度	香农指数	均匀度指数	辛普森指数
平地	对照	2.764±0.033	0.859±0.014	0.916±0.007
	低烈度	2.782±0.045	0.865±0.005	0.920±0.006
	中烈度	2.769±0.053	0.850±0.013	0.916±0.008
	高烈度	2.754±0.031	0.852±0.014	0.916±0.005
坡地	对照	2.626±0.092	0.831±0.015	0.893±0.015
	低烈度	2.813±0.026^*	0.871±0.023^*	0.922±0.003^*
	中烈度	2.772±0.089^***	0.841±0.020	0.914±0.012^***
	高烈度	2.833±0.044^*	0.857±0.009	0.922±0.004