林下仿野生栽培羊肚菌菌丝活动对土壤碳组分与酶活性的空间调控

doi:10.13466/j.cnki.lczyyj.2026.02.007

林草资源研究 ›› 2026›› Issue (2): 70-79.doi: 10.13466/j.cnki.lczyyj.2026.02.007

林下仿野生栽培羊肚菌菌丝活动对土壤碳组分与酶活性的空间调控

彭彬¹(), 魏海霞², 李勋¹(), 张艳¹, 马宏亮¹, 张丹桔³

¹ 四川民族学院横断山区生态保护与特色产业培育四川省教育厅高校重点实验室/甘孜州重点实验室, 四川康定 626001
² 四川农业大学农学院, 成都 611130
³ 四川农业大学林学院, 成都 611130

收稿日期:2026-01-05 修回日期:2026-04-03 出版日期:2026-04-28 发布日期:2026-09-30
通讯作者: 李勋,副教授,主要研究方向为川西地区林草资源与生态保护。Email:502780405@qq.com
作者简介:彭彬,讲师,主要研究方向为特色资源与土壤碳循环。Email:200003@scun.edu.cn
基金资助:
横断山区生态保护与特色产业培育四川省高等学校重点实验室项目“林下复合栽培对菌类农艺性状及土壤酶活性的影响”(HDSQ202410Y006);国家自然科学基金项目“巨桉人工林主效水溶酚类化感物质筛选及生理、生态作用研究”(31770671);四川省科技教育联合基金面上项目“甘孜州林下食用菌栽培对土壤生物群落结构影响的研究”(2024NSFSC2071)

Spatial regulation of soil organic carbon fractions and enzyme activities by Morchella sextelata mycelium activity in an under-forest semi-natural cultivation system

PENG Bin¹(), WEI Haixia², LI Xun¹(), ZHANG Yan¹, MA Hongliang¹, ZHANG Danju³

¹ Key Laboratory of Ecological Protection and Characteristic Industry Cultivation in Hengduan Mountain Area of Sichuan Provincial Department of Education and Ganzi Prefecture, Sichuan Minzu College, Kangding 626001,Sichuan, China
² College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
³ College of Forestry, Sichuan Agricultural University, Chengdu 611130, China

Received:2026-01-05 Revised:2026-04-03 Online:2026-04-28 Published:2026-09-30

摘要/Abstract

摘要：

为揭示羊肚菌菌丝活动对土壤有机碳组分及酶活性的空间影响,以高山松(Pinus densata)林下六妹羊肚菌(Morchella sextelata)仿野生栽培为研究对象,以未栽培为对照(CK),分析距子实体0、20、40 cm处(R、R₂₀、R₄₀)土壤的有机碳组分[易氧化有机碳(ROC)、颗粒态有机碳(POC)、矿物结合态有机碳(MOC)、总有机碳(SOC)]与酶活性[过氧化氢酶(CAT)、脲酶(URE)、β-1,4-葡萄糖苷酶(BG)、纤维二糖水解酶(CBH)]变化,揭示其对土壤碳库及酶活性的空间调控机制。结果表明:1)与CK相比,栽培六妹羊肚菌显著降低了土壤pH(R区域最低)和电导率(EC,R₂₀区域最低);全磷(TP)含量呈下降趋势,全氮(TN)含量呈上升趋势(R₂₀区域最高),但两者均未达到显著水平。2)与CK相比,R的SOC和MOC含量显著降低;R₂₀的SOC及其组分含量(POC、MOC、ROC)均有所提升。3)与CK相比,R的CAT和URE、R₂₀的BG和CBH活性均显著降低;R的BG和CBH活性、R₄₀的CAT和URE活性则均呈上升趋势。4)Mantel检验和冗余分析表明,pH和EC是核心调控因子,直接正向调控土壤酶活性、SOC和MOC,TP则负向调控土壤有机碳组分的转化。羊肚菌菌丝活动对土壤有机碳组分及酶活性的空间分异具有显著调控作用,其调控机制可为仿野生栽培条件下的羊肚菌的可持续经营和土壤健康管理提供理论依据。

关键词: 林下仿野生栽培, 羊肚菌, 菌丝距离, 有机碳组分, 酶活性

Abstract:

To elucidate the spatial effects of Morchella sextelats mycelium activity on soil organic carbon fractions and enzyme activities,under-forest semi-natural cultivation of Morchella sextelata under a Pinus densata forest was investigated,with uncultivated soil serving as the control (CK).Soil samples were collected at distances of 0,20,and 40 cm from the fruiting bodies (R,R₂₀,and R₄₀,respectively).Changes in soil organic carbon fractions [readily oxidizable organic carbon (ROC),particulate organic carbon (POC),mineral-bound organic carbon (MOC),and total organic carbon (SOC)]and enzyme activities [catalase (CAT),urease (URE),β-1,4-glucosidase (BG),and cellobiohydrolase (CBH)]were analyzed to clarify the spatial regulatory mechanisms affecting the soil carbon pool and enzyme activities.The results showed that:1)Compared with CK,cultivation of Morchella sexta.decreased soil pH and electrical conductivity (EC),with the lowest values occurring at R and R20,respectively.Total phosphorus (TP)tended to decrease,whereas total nitrogen (TN) tended to increase and reached its highest value at R₂₀;however,neither change was significant.2)Compared with CK,SOC and MOC contents at R area were significantly lower,whereas SOC and its fractions (POC,MOC,and ROC)at R₂₀ area increased.3)Compared with CK,CAT and URE activities at R a,BG,andCBH activities of R₂₀ were significantly lower.However,BG and CBH activities at R and CAT and URE activities at R₄₀ showed increasing trends.4)Mantel test and redundancy analysis (RDA)showed that pH and EC were the core regulatory factors,exerting direct positive effects on soil enzyme activities,SOC,and MOC,whereas TP negatively regulated the transformation of soil organic carbon fractions.In summary,Morchella sextelata mycelium activity significantly regulated the spatial variation in soil organic carbon fractions and enzyme activity,with pH and EC serving as the key drivers this process.

Key words: under-forest semi-natural cultivation, Morchella, hyphosphere distance, soil organic carbon fractions, enzyme activity

中图分类号:

S714.3

彭彬, 魏海霞, 李勋, 张艳, 马宏亮, 张丹桔. 林下仿野生栽培羊肚菌菌丝活动对土壤碳组分与酶活性的空间调控[J]. 林草资源研究, 2026,(2): 70-79.

PENG Bin, WEI Haixia, LI Xun, ZHANG Yan, MA Hongliang, ZHANG Danju. Spatial regulation of soil organic carbon fractions and enzyme activities by Morchella sextelata mycelium activity in an under-forest semi-natural cultivation system[J]. Forest and Grassland Resources Research, 2026,(2): 70-79.

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区域	pH	EC/(μS/cm)	TP/(mg/g)	TN/(mg/g)
R	7.226±0.020d	178.000±2.309ab	1.853±0.072a	4.389±0.171a
R₂₀	7.312±0.010c	164.667±5.696c	1.723±0.052a	4.417±0.208a
R₄₀	7.371±0.023b	173.333±1.764bc	1.817±0.052a	4.236±0.141a
CK	7.544±0.014a	188.000±2.309a	1.917±0.048a	4.031±0.048a

影响因子	解释率/%	贡献率/%	F值	P值
pH	23.0	36.7	3.4	0.048
EC	21.2	33.7	4.3	0.014
TP	16.6	26.4	2.0	0.154
TN	2.2	3.5	0.4	0.740

林下仿野生栽培羊肚菌菌丝活动对土壤碳组分与酶活性的空间调控

Spatial regulation of soil organic carbon fractions and enzyme activities by Morchella sextelata mycelium activity in an under-forest semi-natural cultivation system

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