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林草资源研究 ›› 2025›› Issue (2): 100-108.doi: 10.13466/j.cnki.lczyyj.2025.02.010

• 科学研究 • 上一篇    下一篇

灌根施用多效唑对沙生植物蒙古羊柴幼苗生理特征的影响

杨蕊1(), 刘阳2, 党晓宏1, 蒙仲举1(), 高永1   

  1. 1.内蒙古农业大学 沙漠治理学院,呼和浩特 010018
    2.内蒙古自治区水利科学研究院,呼和浩特 010020
  • 收稿日期:2025-03-02 修回日期:2025-04-20 出版日期:2025-04-28 发布日期:2025-12-04
  • 通讯作者: 蒙仲举,教授,博士,主要研究方向为水土保持、荒漠化防治、风沙物理、生态修复。Email:mengzhongju@126.com
  • 作者简介:杨蕊,硕士研究生,主要研究方向为水土保持与荒漠化防治。Email:1605122871@qq.com
  • 基金资助:
    国家重点研发计划青年科学家项目“鄂尔多斯盆地植被恢复调控技术研究”(2022YFC3205200);科技领军人才和创新团队建设专项—优青培育项目“风沙采煤沉陷区小叶杨根系萌蘖促发机制”(BR220401)

Effect of root-inoculated paclobutrazol on physiological characteristics of Corethrodendron fruticosum seedlings

YANG Rui1(), LIU Yang2, DANG Xiaohong1, MENG Zhongju1(), GAO Yong1   

  1. 1. College of Desert Control,Inner Mongolia Agricultural University,Hohhot 010018,China
    2. Inner Mongolia Water Conservancy Research Institute,Hohhot 010020,China
  • Received:2025-03-02 Revised:2025-04-20 Online:2025-04-28 Published:2025-12-04

摘要: 多效唑(PP333)是一种赤霉素抑制剂,能够调控植物的生理过程并提高其抗逆性。为探讨蒙古羊柴幼苗生理过程对PP333的响应,以蒙古羊柴幼苗为试材,采用盆栽法,通过150、300、450、600 mg/L 4个质量浓度的PP333溶液灌根处理,分析其对幼苗光合、抗氧化及渗透调节能力的影响。结果表明:150~600 mg/L质量浓度的PP333溶液灌根处理能够提高蒙古羊柴幼苗的光合、抗氧化及渗透调节能力;随着PP333溶液质量浓度的增加,蒙古羊柴幼苗的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)等光合气体交换参数以及光合色素含量、抗氧化酶活性、渗透调节物质等生理指标,整体呈现出先升高后降低的变化规律,并于450 mg/L质量浓度的PP333溶液灌根处理下达到最优后下降,而胞间CO2摩尔分数(Ci)及丙二醛(MDA)含量在相同条件下呈现出相反规律。综上,150~600 mg/L质量浓度的PP333溶液灌根处理能够通过缓解蒙古羊柴幼苗光合作用的非气孔限制、减少膜脂过氧化产物的积累,提高光合能力、生物膜保护能力及抗氧化能力,促进渗透物质积累,增强蒙古羊柴幼苗的抗逆性。其中,以450 mg/L质量浓度的PP333灌根处理效果最优。

关键词: 蒙古羊柴幼苗, 多效唑, 生理特征, 抗逆性

Abstract:

Paclobutrazol(PP333)is a gibberellin inhibitor that can regulate plant physiological processes and enhance their stress tolerance.In order to investigate the physiological responses of Corethrodendron fruticosum seedlings to PP333,potted method was used to study the effects of root inoculated PP333 with different mass concentrations(150,300,450,and 600 mg/L)on photosynthesis,antioxidant and osmotic regulation abilities of C.fruticosum seedlings.The results showed that root irrigation treatment with 150~600 mg/L mass concentration of PP333 improved photosynthesis,antioxidant and osmotic regulation abilities of C.fruticosum seedlings.With the increase of PP333 mass concentration,the photosynthetic gas exchange parameters such as net photosynthetic rate(Pn),transpiration rate (Tr),stomatal conductance(Gs),photosynthetic pigment content,antioxidant enzyme activity and osmotic adjustment substances of C.fruticosum seedlings showed a trend of increasing first and then decreasing,and decreased after reaching the optimal inflection point under the treatment of 450 mg/L PP333,while intercellular CO2 mole fraction(Ci)and malondialdehyde content(MDA)showed the opposite trend under the same conditions.In conclusion,root irrigation treatment with 150~600 mg/L mass concentration of PP333 could improve photosynthesis,plasma membrane protection and antioxidant capacity,accumulate osmotic substances,and enhance the stress resistance of C.fruticosum seedlings by alleviating non-stomatal photosynthesis and reducing the accumulation of membrane lipid peroxidation products on C.fruticosum seedlings,450 mg/L of PP333 root irrigation treatment showed the best effect.

Key words: Corethrodendron fruticosum seedlings, paclobutrazol(PP333), physiological characteristics, stress resistance

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