Regularity and Physiological Characteristics of Leaf Color Change During the Growth Period of Quercus mongolica

doi:10.13466/j.cnki.lczyyj.2024.05.013

Abstract

Abstract:

This study investigates the alterations in physiological substances during the leaf coloration of Quercus mongolica,aiming to provide foundational data for breeding new varieties and enriching the genetic resources of colored-leaf tree species.Using young leaf stage(S1),green leaf stage(S2-S4),color changing stage(S5),and red leaf stage(S6)leaves as test materials.Measurement of changes in leaf pigments,leaf color parameters,osmoregulatory substances,nitrogen(N),phosphorus(P),antioxidant enzymes,and MDA at different stages.This study investigates the alterations in physiological substances during the leaf coloration of Q.mongolica,aiming to provide foundational data for breeding new varieties and enriching the genetic resources of colored-leaf tree species.1)During the color changing stage of leaves,the contents of chlorophyll decreased,while the content of carotenoids increased and anthocyanins accumulated in large quantities.Carotenoid to chlorophyll ratio and anthocyanin to chlorophyll ration showed an upward trend,while anthocyanin to carotenoid ratio showed little change.2)The leaf color parameter a^* showed a significant upward trend,while L^*,b^*,and C first decreased and then increased.The color changing stage of hue(H)and saturation(s)values increased sharply,while hue angle(h)decreased significantly.3)The N and P contents of Q.mongolicum leaves decreased overall,while the proline content increased.The soluble protein content decreased during color changing stage,and soluble sugars increased.The change range of Peroxidase(POD) was small,while Superoxide dismutase(SOD) showed a gentle upward trend.Catalase(CAT)activity and malondialdehyde(MDA)levels increased significantly during the color changing stage.4)Anthocyanins were highly significantly positively correlated with a^*,and together they were highly significantly positively correlated with proline,anthocyanins to chlorophyll ratio.There was a significant positive correlation with MDA and SOD,a significant negative correlation with N,chlorophyll,and a negative correlation with P.5)Among the three principal components,principal component 1 had the highest eigenvalue and contribution rate,and the eigenvectors of anthocyanins to chlorophyll ratio are the largest.Chlorophyll degradation,anthocyanin accumulation,and a significant increase in the anthocyanin to chlorophyll ratio were identified as the primary physiological factors influencing the red coloration of Q.mongolicum leaves.During the color changing stage,the content of proline and soluble sugars increased,while the content of N and P decreased,and the content of MDA increased,and the activities of SOD and CAT were enhanced,synergistically promoting anthocyanin synthesis and leaf coloration.

Key words: Quercus mongolica, leaf color, anthocyanins, chlorophyll, color changing stage

CLC Number:

S792.186

YUAN Yangchen, PANG Jiushuai, YU haidong, ZHANG jiang, CHEN huimin, ZHANG bingying, LIU jialin, HUO wei. Regularity and Physiological Characteristics of Leaf Color Change During the Growth Period of Quercus mongolica[J]. Forest and Grassland Resources Research, 2024, (5): 116-127.

Figures/Tables 8

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Tab.1

References 42

[1]	罗安才, 阎晓灵, 李利霞, 等. 鸡爪槭叶色变化机制研究[J]. 江苏农业科学, 2018, 46(22):118-122.
[2]	唐生森, 陈虎, 覃永康, 等. 枫香秋季变色期叶色变化及其生理基础[J]. 广西植物, 2021, 41(12):2061-2068.
[3]	SCHABERG G P, MURAKAMI F P, TUMER R M, et al. Association of red coloration with senescence of sugar maple leaves in autumn[J]. Trees Structure and Function, 2008, 22(4):573-578.
[4]	TIAN Yueyue, WANG Hanyue, SUN Ping, et al. Response of leaf color and the expression of photoreceptor genes of Camellia sinensis cv.Huangjinya to different light quality conditions[J]. Scientia Horticulturae, 2019,251:225-232.
[5]	李卫星, 杨舜博, 何智冲, 等. 植物叶色变化机制研究进展[J]. 园艺学报, 2017, 44(9):1811-1824. doi: 10.16420/j.issn.0513-353x.2017-0167
[6]	孙健. 4种彩叶植物生长季叶色表达与色素含量关系研究[D]. 呼和浩特: 内蒙古农业大学, 2022.
[7]	ROLLAND F, BAENA-GONZALEZ E, SHEEN J. Sugar sensing and signaling in plants:Conserved and novel mechanisms[J]. Annual Review of Plant Biology, 2006,57:675-709.
[8]	梁玲, 江洁蓓, 张腾驹, 等. 不同色彩珙桐苞片与叶片的生理特性研究[J]. 植物研究, 2020, 40(4):505-513. doi: 10.7525/j.issn.1673-5102.2020.04.004
[9]	MIN Ting, XIE Jun, ZHENG Menglin, et al. The effect of different temperatures on browning incidence and phenol compound metabolism in fresh-cut lotus(Nelumbo nucifera G.) root[J]. Postharvest Biology and Technology, 2017,123:69-76.
[10]	BECKER C, KLAERING H P, KROH L W, et al. Cool-cultivated red leaf lettuce accumulates cyanidin-3-O-(6-O-malonyl)-glucoside and caffeoylmalic acid[J]. Food Chemistry, 2014,146:404-411.
[11]	刘万德, 苏建荣, 李帅锋, 等. 云南普洱季风常绿阔叶林优势物种不同生长阶段叶片碳、氮、磷化学计量特征[J]. 植物生态学报, 2015, 39(1):52-62. doi: 10.17521/cjpe.2015.0006
[12]	李一, 潘立本, 赵雯, 等. 蒙古栎叶片功能性状变化特征及影响因素[J]. 东北林业大学学报, 2024, 52(3):10-15.
[13]	原阳晨, 庞久帅, 周苗苗, 等. 华北平原区蒙古栎幼苗栽培条件研究[J]. 林业与生态科学, 2023, 38(2):233-240. doi: 10.13320/j.cnki.hjfor.2023.0030
[14]	李佳宁. 蒙古栎生殖生物学特性的初步研究[D]. 沈阳: 沈阳农业大学, 2020.
[15]	YUAN Yangchen, ZHANG Weiqiang, PANG Jiushuai, et al. Integrated physiological and metabolomic analyses reveal changes during the natural senescence of Quercus mongolica leaves[J]. PLoS One, 2023,18:e0289272.
[16]	王学奎. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社,2006:134-137.
[17]	宋岩. 美国红枫和元宝枫呈色的生理特性研究[D]. 沈阳: 沈阳农业大学, 2018.
[18]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[19]	周航宇, 付琪瑶, 梁婉婷, 等. 天然油松叶-枝-根氮磷含量随降水和温度的变化规律[J]. 北京林业大学学报, 2024, 46(1):44-54.
[20]	黄秋婵, 韦友欢, 韦方立, 等. 常见夹竹桃科植物叶片颜色及类胡萝卜素含量的比较分析[J]. 安徽农业科学, 2011, 39(25):15203-15204.
[21]	HUGHES N M. Winter leaf reddening in ‘evergreen’ species[J]. The New Phytologist, 2011, 190(3):573-581.
[22]	LINDA C S. Environmental significance of an-thocyanins in plant stress responses[J]. Photochenistry and Photobiology, 1999, 70(1):1-9.
[23]	陈倩如, 蔡文淇, 张霞, 等. 檵木不同叶色形成的化学成分比较研究[J]. 园艺学报, 2021, 48(10):1969-1982. doi: 10.16420/j.issn.0513-353x.2021-0815
[24]	楚爱香, 张要战, 田永芳. 几种秋色叶树种秋冬转色期叶色变化的生理特性[J]. 东北林业大学学报, 2012, 40(11):40-43.
[25]	江洁蓓, 梁玲, 张腾驹, 等. 粉红珙桐叶片呈色相关生理特性的季节变化[J]. 西北植物学报, 2019, 39(11):2019-2027.
[26]	潘丽芹, 李纪元, 韦海忠, 等. 红叶山茶(Camellia japonica)叶片色素含量与叶色参数的相关性分析[J]. 分子植物育种, 2020, 18(2):579-585.
[27]	许志钊, 杨秀云, 王祎琛, 等. 黄连木变色期叶片色素变化规律及呈色机理[J]. 南京林业大学学报(自然科学版), 2024, 48(2):97-104. doi: 10.12302/j.issn.1000-2006.202210034
[28]	齐睿, 李小红, 石博雨, 等. 红叶石楠转色期叶片色彩参数与色素含量的相关性分析[J]. 河南农业科学, 2019, 48(4):93-101.
[29]	王嘉佳, 唐中华. 可溶性糖对植物生长发育调控作用的研究进展[J]. 植物学研究, 2014(3):71-76.
[30]	王燕龙, 车晓雨, 李彦慧, 等. 狭叶白蜡秋季叶片呈色生理变化研究[J]. 河北农业大学学报, 2023, 46(4):55-64.
[31]	姜琳, 杨暖, 姜官恒, 等. 栎属4个树种秋冬叶色与生理变化的关系[J]. 中国农学通报, 2015, 31(19):13-18. doi: 10.11924/j.issn.1000-6850.casb15010198
[32]	楚爱香, 张要战, 王萌萌. 四种槭树属(Acer)植物秋色叶变化与色素含量和可溶性糖的关系[J]. 江西农业大学学报, 2013, 35(1):108-111.
[33]	聂庆娟, 史宝胜, 孟朝, 等. 不同叶色红栌叶片中色素含量、酶活性及内含物差异的研究[J]. 植物研究, 2008(5):599-602. doi: 10.7525/j.issn.1673-5102.2008.05.020
[34]	全先庆, 张渝洁, 单雷, 等. 脯氨酸在植物生长和非生物胁迫耐受中的作用[J]. 生物技术通讯, 2007(1):159-162.
[35]	左师宇. 脯氨酸提高玉米耐低温胁迫的生理机理研究[D]. 哈尔滨: 东北农业大学, 2022.
[36]	安然, 王树凤, 陈益泰, 等. 栎树秋季叶色多样性及相关生理特性的变化[J]. 林业科学研究, 2022, 35(2):78-88.
[37]	李顺. 环境因子和系统发育对植物叶片养分重吸收的影响[D]. 大理: 大理大学, 2023.
[38]	OUGHAM J H, MORRIS P, THOMAS H. The colors of autumn leaves as symptoms of cellular recycling and defenses against environmental stresses[J]. Current Topics in Developmental Biology, 2005,66:135-160.
[39]	陈全战, 张边江, 周峰, 等. 等渗盐分、水分胁迫对番茄幼苗生理指标的影响[J]. 湖北农业科学, 2008(5):525-527.
[40]	ZHOU Jie, WANG Jian, SHI Kai, et al. Hydrogen peroxide is involved in the cold acclimation-induced chilling tolerance of tomato plants[J]. Plant Physiology and Biochemistry, 2012,60:141-149.
[41]	MISHRA P, BHOOMIKA K, DUBEY R S. Differential responses of antioxidative defense system to prolonged salinity stress in salt tolerant and salt sensitive Indica rice(Oryza sativa L.) seedlings[J]. Protoplasma, 2013,250:3-19.
[42]	李瑞雪. 抗寒常绿木兰科植物的筛选及抗寒机理研究[D]. 长沙: 中南林业科技大学, 2018.

生理指标	第1 主成分	第2 主成分	第3 主成分
叶绿素a(Chla)	-0.080	0.120	-0.036
叶绿素b(Chlb)	-0.082	0.122	-0.050
叶绿素(Chl)	-0.081	0.121	-0.046
类胡萝卜素(Car)	0.070	-0.187	0.006
花青素(Ant)	0.087	0.066	0.025
类胡萝卜素/叶绿素(Car/Chl)	0.080	-0.133	-0.046
花青素/叶绿素(Ant/Chl)	0.089	-0.019	-0.024
花青素/类胡萝卜素(Ant/Car)	0.039	0.278	-0.005
氮(N)	-0.077	-0.138	0.115
磷(P)	-0.057	-0.226	0.138
脯氨酸(Pro)	0.088	-0.010	0.072
可溶性蛋白(Sp)	0.048	0.231	-0.108
可溶性糖(Ss)	0.065	0.042	0.346
过氧化物酶(POD)	0.041	0.089	0.428
过氧化氢酶(CAT)	0.052	-0.101	-0.351
超氧化物歧化酶(SOD)	0.081	0.009	0.016
丙二醛(MDA)	0.082	0.028	-0.181
特征值	11.233	3.099	1.930
贡献率/%	66.195	18.231	11.352
累计贡献率/%	66.195	84.462	95.778