Effects of light environments within Phoebe bournei canopy on leaf structural traits and photosynthetic induction

doi:10.13466/j.cnki.lczyyj.2025.02.008

Abstract

Abstract:

To investigate the mechanism by which the canopy light environment influences leaf structure and photosynthetic induction characteristics in Phoebe bournei,we examined the effects of contrasting light environments on the morphological traits,stomatal traits,photosynthesis-light response and photosynthetic induction of sun(high-light environments)and shade leaves(low-light environments)at different canopy positions in canopy of 22-year P.bournei.The results showed that:1)The leaf thickness,palisade tissue thickness,spongy tissue thickness,stomatal density,and stomatal relative area of leaves in the basal and upper part of P.bournei canopy were decreased with the reduction of canopy light intensity.Compared with the high-light environment,the low-light environment reduced the leaf mass per area,maximum net photosynthetic rate,light compensation point,and light saturation point of the basal and upper leaves of canopy.2)Under a photosynthetically available radiation of 1 000 μmol/(m²·s),low light significantly decreased(P<0.05)the net photosynthetic rate(P_{n 1000}),electron transfer rate(J_{1 000})and maximum carboxylation rate of Rubisco(V_cmax)in the basal and upper leaves of canopy.3)During photosynthetic induction,leaves in the high-light environment achieved 90% of P_{n 1000} (t₉₀_P_n)significantly faster(P<0.05)than those in the low-light environment.Notably,t₉₀_P_n was a significantly negatively correlated with stomatal density,leaf thickness,palisade tissue thickness,and spongy tissue thickness.In conclusion,P.bournei adapts to high-light conditions by enhancing photosynthetic carbon fixation capacity through increased apparent quantum yield,light compensation point,Rubisco carboxylation rate and electron transfer rate.Simultaneously,P.bournei enhances its photosynthetic induction rate by increasing stomatal density and promoting rapid stomatal opening during the late stage of photosynthetic induction,thereby enhancing its ability to utilize light patches.

Key words: Phoebe bournei canopy, light environment, leaf structure, photosynthesis, photosynthetic induction

CLC Number:

S711

LUO Congcong, HUANG Wenchao, LIU Xinliang, YE Tiantian, LIU Linqi, TANG Xinglin. Effects of light environments within Phoebe bournei canopy on leaf structural traits and photosynthetic induction[J]. Forest and Grassland Resources Research, 2025, (2): 79-88.

Figures/Tables 6

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References 33

[1]	骆丹, 王春胜, 曾杰. 西南桦幼林冠层光合特征及其对造林密度的响应[J]. 中南林业科技大学学报, 2020, 40(4):44-49.
[2]	刘晓东, 朱春全, 雷静品, 等. 杨树人工林冠层光合辐射分布的研究[J]. 林业科学, 2000, 36(3):2-7.
[3]	TANG Xinglin, LIU Guangzheng, JIANG Jiang, et al. Effects of growth irradiance on photosynthesis and photorespiration of Phoebe bournei leaves[J]. Functional Plant Biology, 2020, 47(12):1053-1061. doi: 10.1071/FP20062
[4]	冷寒冰, 万宁海, 刘群录. 香樟冠层光环境对叶片功能性状和光合特性的影响[J]. 应用生态学报, 2023, 34(8):2113-2122. doi: 10.13287/j.1001-9332.202308.027
[5]	YANG Feng, FENG Lingyang, LIU Qinlin, et al. Effect of interactions between light intensity and red-tofar-red ratio on the photosynthesis of soybean leaves under shade condition[J]. Environmental and Experimental Botany, 2018,150:79-87.
[6]	李理渊, 李俊, 同小娟, 等. 黄河小浪底栓皮栎、刺槐叶片电子传递速率光响应的模拟[J]. 植物生态学报, 2018, 42(10):1009-1021. doi: 10.17521/cjpe.2018.0063
[7]	COBLE A P, CAVALERI M A. Light drives vertical gradients of leaf morphology in a sugar maple (Acer saccharum)forest[J]. Tree physiology, 2014, 34(2):146-158. doi: 10.1093/treephys/tpt126
[8]	马雅莉, 郭素娟. 板栗冠层光合特性的空间异质性研究[J]. 北京林业大学学报, 2020, 42(10):71-83.
[9]	SAKODA K, YAMORI W, GROSZMANN M, et al. Stomatal,mesophyll conductance,and biochemical limitations to photosynthesis during induction[J]. Plant Physiology, 2021,185:146-160.
[10]	SHIMADZU S J, SEO M, TERASHIMA I, et al. Whole irradiated plant leaves showed faster photosynthetic induction than individually irradiated leaves via improved stomatal opening[J]. Frontiers in Plant Science, 2019,10:1512.
[11]	KRÜGER G H J, TSIMILLI-MICHAEL M, STRASSER R J. Light stress provokes plastic and elastic modifications in structure and function of photosystem II in camellia leaves[J]. Physiologia Plantarum, 1997, 101(2):265-277. doi: 10.1111/ppl.1997.101.issue-2
[12]	URBAN O, KOŠVANCOVÁ M, MAREK M V, et al. Induction of photosynthesis and importance of limitations during the induction phase in sun and shade leaves of five ecologically contrasting tree species from the temperate zone[J]. Tree Physiology, 2007, 27(8):1207-1215. pmid: 17472946
[13]	ZHANG Ningyi, BERMAN S R, JOUBERT D, et al. Variation of photosynthetic induction in major horticultural crops is mostly driven by differences in stomatal traits[J]. Frontiers in Plant Science, 2022,13:860229.
[14]	TANAKA Y, ADACHI S, YAMORI W. Natural genetic variation of the photosynthetic induction response to fluctuating light environment[J]. Current Opinion in Plant Biology, 2019,49:52-59.
[15]	胡彦波, 许楠, 包卓, 等. 桑树叶片光合诱导对光强转换的响应[J]. 中国生态农业学报, 2010, 18(4):799-803.
[16]	林智榕, 李成珺, 陈水兰, 等. 杉木闽楠异龄复层林中闽楠叶片形态适应特征[J]. 森林与环境学报, 2024, 44(2):113-119.
[17]	游景晖, 欧阳勋志, 李坚锋, 等. 闽楠天然次生林不同林层碳密度变化特征及其影响因素[J]. 东北林业大学学报, 2024, 52(4):89-94.
[18]	薛黎, 李志辉, 童方平, 等. 遮阴对闽楠幼苗光合及其叶片解剖特性的影响[J]. 西北植物学报, 2019, 39(7):1221-1229.
[19]	LIU Congcong, HE Nianpeng, ZHANG Jiahui, et al. Variation of stomatal traits from cold temperate to tropical forests and association with water use efficiency[J]. Functional Ecology, 2018,32:20-28.
[20]	张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学通报, 1999(4):444-448.
[21]	YE Zipiao, SUGGETT D J, ROBAKOWSKI P, et al. A mechanistic model for the photosynthesis-light response based on the photosynthetic electron transport of photosystem II in C₃ and C₄ species[J]. New Phytologist, 2013,199:110-120.
[22]	DE KAUWE M G, LIN Yanshi, WRIGHT I J, et al. A test of the 'one-point method' for estimating maximum carboxylation capacity from field-measured,light-saturated photosynthesis[J]. New Phytologist, 2016,210:1130-1144.
[23]	KAISER E, KROMDIJK J, HARBINSON J, et al. Photosynthetic induction and its diffusional,carboxylation and electron transport processes as affected by CO₂ partial pressure,temperature,air humidity and blue irradiance[J]. Annals of Botany, 2017, 119(1):191-205. doi: 10.1093/aob/mcw226
[24]	ZHANG Ningyi, BERMAN S R, VAN DEN BERG T, et al. Biochemical versus stomatal acclimation of dynamic photosynthetic gas exchange to elevated CO₂ in three horticultural species with contrasting stomatal morphology[J]. Plant Cell and Environment, 2024,47:4516-4529.
[25]	薛姗姗, 郑林, 李璧滢, 等. 环境因子调控气孔发育的分子机制[J]. 植物生理学报, 2024, 60(9):1375-1389.
[26]	MASLOVA N P, KARASEV E V, KODRUL T M, et al. Sun and shade leaf variability in Liquidambar chinensis and Liquidambar formosana(Altingiaceae):Implications for palaeobotany[J]. Botanical Journal of the Linnean Society, 2018,188:296-315.
[27]	袁媛, 徐克芹, 曾平生, 等. 亚热带生态系统5种修复树种的光合特征[J]. 东北林业大学学报, 2024, 52(5):28-33.
[28]	唐星林, 姜姜, 金洪平, 等. 遮阴对闽楠叶绿素含量和光合特性的影响[J]. 应用生态学报, 2019, 30(9):2941-2948. doi: 10.13287/j.1001-9332.201909.002
[29]	李林宽, 张锡洲, 陈静, 等. 缺磷限制带状复合种植大豆光合作用对光照诱导的响应[J]. 植物营养与肥料学报, 2024, 30(3):505-514.
[30]	LIU Qiuping, JIN Wei, HUANG Liying, et al. Photosynthetic induction characteristics in saplings of four sun-demanding trees and shrubs[J]. Plants-basel, 2025, 14(1):144.
[31]	CHEN Yue, XU Daquan. Two patterns of leaf photosynthetic response to irradiance transition from saturating to limiting one in some plant species[J]. New Phytologist, 2006,169:789-798.
[32]	KANG Huixing, TOMIMATSU H J, ZHU Ting, et al. Contributions of species shade tolerance and individual light environment to photosynthetic induction in tropical tree seedlings[J]. Tree Physiology, 2022(10):1975-1987.
[33]	ZHUANG Xiong, DUN Zhigang, WANG Yucheng, et al. Effect of stomatal morphology on leaf photosynthetic induction under fluctuating light in Rice[J]. Frontiers in Plant Science, 2022,12:754790.

参数	冠层基部阳生叶	冠层顶部阳生叶	冠层基部阴生叶	冠层顶部阴生叶
叶片厚度/μm	159.80±9.60ab	180.20±4.10a	121.80±5.70c	130.80±5.90bc
上表皮厚度/μm	13.80±1.02ab	16.40±0.83a	12.30±0.44b	12.20±0.37b
下表皮厚度/μm	13.70±0.42ab	14.60±0.74a	11.80±0.29b	13.00±0.61ab
栅栏组织厚度/μm	83.70±4.41ab	85.00±1.71a	58.80±4.08c	66.90±4.23bc
海绵组织厚度/μm	50.50±3.68ab	62.70±6.55a	41.20±3.38b	42.00±2.96b
栅海比	1.66±0.04a	1.39±0.15a	1.43±0.05a	1.60±0.09a
气孔大小/μm²	151.50±4.00a	155.00±2.90a	149.70±0.30a	153.60±4.90a
气孔密度/(个/mm²)	932.20±33.30a	970.30±5.30a	703.60±25.40b	773.90±13.40b
气孔相对面积/(μm²/mm²)	14.10±0.40a	15.00±0.29a	10.50±0.40b	11.90±0.50b

参数	冠层基部阳生叶	冠层顶部阳生叶	冠层基部阴生叶	冠层顶部阴生叶
叶绿素相对含量	41.90± 0.50b	40.70± 0.30b	46.00± 0.40a	44.30± 0.40a
比叶质量/(g/m²)	137.30± 2.07a	133.60± 2.76a	83.70±10.25b	95.40± 2.77b
表观量子效率/(mmol/mol)	0.032± 0.002ab	0.034± 0.000 3a	0.024± 0.003bc	0.023± 0.004c
最大净光合速率^①/[μmol/(m²·s)]	7.16± 0.09a	7.07± 0.30ac	3.50± 0.42bc	3.49± 0.56b
暗呼吸速率/[μmol/(m²·s)]	0.49± 0.02b	0.78± 0.06a	0.40± 0.12b	0.33± 0.07b
光补偿点/[μmol/(m²·s)]	9.61± 0.46b	15.57± 0.97a	5.55± 1.62c	5.07± 1.05c
光饱和点^①/[μmol/(m²·s)]	757.30±14.82ab	810.50±26.33a	541.50±10.18c	650.10±112.48bc
初始斜率^①	0.340± 0.008bc	0.320± 0.002c	0.420± 0.003a	0.410± 0.007ab
最大电子传递速率^①/[μmol/(m²·s)]	116.60±15.37a	122.70± 7.94a	46.40± 0.24b	49.60± 3.77ab
饱和光强^①/[μmol/(m²·s)]	824.70±71.61ab	888.90±59.45a	540.80±4.61c	578.40± 0.58bc

参数		冠层基部阳生叶		冠层顶部阳生叶		冠层基部阴生叶		冠层顶部阴生叶
P_{n 50}/[μmol/(m²·s)]		1.33±0.23a		1.32±0.06a		1.19±0.13a		1.40±0.32a
P_{n 1000}/[μmol/(m²·s)]		6.66±0.21a		6.77±0.27a		3.80±0.32b		3.50±0.43b
G_s50/[μmol/(m²·s)]		20.30±4.40a		21.40±1.40a		11.40±2.70a		11.10±1.60a
G_{S 1000}^①/[μmol/(m²·s)]		66.10±4.20ab		69.60±5.60a		30.60±1.90b		30.10±2.20b
J₅₀/[μmol/(m²·s)]		15.20±0.20a		15.20±0.20a		15.00±0.10a		14.90±0.10a
J_{1 000}/[μmol/(m²·s)]		106.90±12.00a		131.00±4.40a		46.10±4.10b		54.00±6.70b
V_cmax/[μmol/(m²·s)]		158.20±24.20a		201.10±6.20a		63.40±8.70b		77.90±10.70b
t₅₀_Pn/min		14.70±0.30a		14.30±1.30a		18.00±1.50a		17.70±1.20a
t₉₀_Pn/min		22.00±1.00b		23.00±1.50b		31.70±0.90a		35.00±1.50a
t_50Gs^① /min		17.00±0.60a		17.00±0.10a		20.70±2.90a		17.30±1.70a
t_90Gs^①/min	26.30±2.80a		26.00±2.10a		34.30±5.70a		27.30±6.40a
t₅₀_J/min	11.30±0.30a		10.70±0.70a		10.30±0.30a		9.30±0.30a
t₉₀_J/min	21.30±0.90a		21.30±1.50a		20.70±2.30a		21.70±1.30a