Effects of Nitrogen Application and Mixed Planting of Nitrogen-Fixing Tree Species on Phosphorus Distribution and Growth of Eucalyptus urophylla×grandis

doi:10.13466/j.cnki.lczyyj.2024.04.015

Abstract

Abstract:

Mixing nitrogen-fixing tree species with Eucalyptus urophylla×grandis can enhance soil nitrogen(N)content.However,excessive N may disrupt the N:P ratio and intensify phosphorus(P)limitation for Eucalyptus urophylla×grandis.Understanding the effects of N-fixing tree species on soil N and P content and Eucalyptus urophylla×grandis P nutrients is essential for sustainable nutrient management in Eucalyptus urophylla×grandis plantations.This study included three treatments:Eucalyptus urophylla×grandis mixed with N-fixing species(ME),pure Eucalyptus urophylla×grandis plantation with N application(NE)and pure Eucalyptus urophylla×grandis plantation without fertilization(PE).And this study analyze the differences and seasonal dynamics of soil N and P levels,Eucalyptus urophylla×grandis biomass,P concentration and P accumulation in Eucalyptus urophylla×grandis plants.The results showed that both ME and NE treatments significantly increased soil total N content(from 36% to 46%),available N content(from 20% to 40%),Eucalyptus urophylla×grandis biomass(from 16% to 23%)and P accumulation(from 16% to 36%)compared to the PE treatment.In the ME treatment,the soil available N:P ration remained balanced,whereas it significantly increased in the NE treatment.The P concentration in Eucalyptus urophylla×grandis organs showed the order of ME>PE>NE.Correlation analysis revealed that leaf P concentration was influenced by soil available P(16.42%)and the soil available N:P ratio(15.13%).Both N application and introduction of N-fixing tree species treatments significantly increased soil N content.However,N application decreased phosphorus concentration and AP in all organs of Eucalyptus urophylla×grandis,which aggravated the phosphorus limitation in the later stage of Eucalyptus urophylla×grandis.The mixed treatment of nitrogen-fixing tree species maintained the balance of AP content and the AN:AP ration,alleviating N and P limitations and promoting long-term Eucalyptus urophylla×grandis growth.In the short term,introducing nitrogen-fixing tree species improved soil N and P stability and promoted Eucalyptus urophylla×grandis growth.

Key words: mixed forest, nitrogen-fixing tree species, phosphorus concentration, biomass

CLC Number:

LI Zihua, YAO Xianyu, LIAO Zihua, XIONG Junfei, YE Shaoming. Effects of Nitrogen Application and Mixed Planting of Nitrogen-Fixing Tree Species on Phosphorus Distribution and Growth of Eucalyptus urophylla×grandis[J]. Forest and Grassland Resources Research, 2024, (4): 135-146.

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指标	pH	TOC	TN	TP	AN	AP	AN:TN/%	AP:TP/%	N:P	AN:AP
处理F值	7.86^*	8.24^*	19.27^*	3.79^*	25.45^*	5.40^*	0.69	3.79^*	14.7^*	7.59^*
季节F值	0	11.65^*	21.15^*	12.78^*	10.4^*	1.31	3.2	0.16	22.65^*	6.27^*
处理*季节F值	0.15	0.23	0.24	0.18	1.38	1.55	0.61	1.57	0.44	0.98
指标	细根生物量	中根生物量	粗根生物量	巨根生物量	干材生物量	干皮生物量	树枝生物量	树叶生物量	全株生物量
处理F值	11.45^*	4.78^*	26.4^*	8.94^*	23.94^*	23.33^*	15.36^*	0.63	34.23^*
季节F值	3.4	10.54^*	38.56^*	5.63^*	10.18^*	0.11	4.26^*	1.34	7.13^*
处理*季节F值	0.35	2.37	3.81^*	0.33	0.24	1.31	1.9	0.19	0.39
指标	细根磷浓度	中根磷浓度	粗根磷浓度	巨根磷浓度	干材磷浓度	干皮磷浓度	树枝磷浓度	树叶磷浓度
处理F值	9.10^*	1.23	1.11	5.07^*	8.72^*	21.29^*	4.87^*	24.27^*
季节F值	29.74^*	0.07	0.4	0.45	1.11	1.29	28.37^*	18.45^*
处理*季节F值	1.37	0.26	0.04	0.03	0.11	0.48	0.58	0.86
指标	细根磷积累量	中根磷积累量	粗根磷积累量	巨根磷积累量	干材磷积累量	干皮磷积累量	树枝磷积累量	树叶磷积累量	全株磷积累量
处理F值	16.73^*	3.01	9.46^*	5.93^*	13.55^*	23.57^*	13.59^*	7.08^*	33.70^*
季节F值	2.12	7.03^*W	9.04^*	1.07	1.54	0.53	1.01	1.67	2.74
处理*季节F值	1.1	1.94	1.47	0.2	0.37	0.46	0.7	0.5	0.73

季节	处理	PH	TOC/(g/kg)	TN/(g/kg)	TP/(mg/kg)	AN/(mg/kg)	AP/(mg/kg)	AN:TN/%	AP:TP/%	N:P	AN:AP
秋季	ME	4.20±0.21A	19.96±1.02A	1.61±0.17A	467.87±13.62A	16.92±1.50A	8.13±1.55A	1.06±0.18A	1.74±0.36A	3.45±0.43A	2.16±0.54B
	NE	3.93±0.21A	18.38±1.72AB	1.69±0.31A	441.71±22.81B	15.35±1.14A	5.19±1.32B	0.94±0.22A	1.17±0.29B	3.85±0.86A	3.15±0.93A
	PE	4.15±0.23A	16.38±1.99B	1.18±0.13B	462.37±16.48AB	12.71±1.46B	6.55±1.00AB	1.09±0.19A	1.41±0.19AB	2.55±0.31B	1.96±0.28B
春季	ME	4.23±0.11a	17.67±2.04a	1.33±0.17a	495.71±18.28a	14.59±0.89a	7.86±1.63a	1.11±0.12a	1.59±0.34a	2.68±0.36a	1.93±0.44ab
	NE	3.87±0.24b	15.58±1.43ab	1.31±0.13a	472.63±27.53a	14.90±1.66a	6.94±1.71a	1.15±0.21a	1.47±0.36a	2.79±0.42a	2.26±0.60a
	PE	4.17±0.11a	14.69±2.34b	0.91±0.11b	482.77±19.55a	10.64±1.46b	6.75±0.35a	1.18±0.08a	1.40±0.10a	1.88±0.22b	1.57±0.16b