复合生长调节剂(IAA+NAA)对降香黄檀扦插繁殖的调控效应

doi:10.13466/j.cnki.lczyyj.2025.05.006

摘要/Abstract

摘要：

为优化降香黄檀(Dalbergia odorifera)扦插繁殖技术体系、解决其生根效率低下的技术问题,采用双因素试验,探究不同浓度(200、400、600、800 mg/L)和浸泡时间(10 s、1 min、30 min、60 min)下复合生长调节剂(IAA+NAA)对降香黄檀扦插繁殖效果的影响。结果表明:1)激素浓度、浸泡时间及二者交互作用对降香生根率、根干质量、总根长等指标均具有极显著影响(P<0.01)。激素浓度对多项指标均具有非线性调控效应,其中400 mg/L处理在多项指标中表现最优:400 mg/L+10 s处理的生根率达92.5%,根干质量为0.035 g,最大根长及新梢干质量亦显著优于其他处理。2)浸泡时间对生根的影响具有浓度依赖性:低浓度(200 mg/L)下延长时间可促进生根,而中高浓度下短时处理(10 s~1 min)更优,长时间浸泡(60 min)则显著抑制生长。3)相关性分析表明,生根率与最大根长、总根长、新梢数及新梢干质量均呈极显著正相关(P<0.01),与根干质量呈显著正相关(P<0.05),根干质量与新梢干质量也呈极显著相关,表明根系与地上部分生长之间存在协同促进关系。4)主成分分析表明,400 mg/L+10 s为最优处理,其得分显著高于其他组合。明确“中浓度-短时间”为降香黄檀扦插繁殖的高效策略,为该树种的规模化无性繁殖提供了理论依据与技术参数。

关键词: 降香黄檀, 扦插繁殖, 生长调节剂, 生根率, 新梢发育

Abstract:

To optimize the cutting propagation technical system of Dalbergia odorifera and resolve the technical bottleneck of its low rooting efficiency,a two-factor experiment was conducted to investigate the effects of compound growth regulators(IAA+NAA)on its cutting propagation.The results demonstrated that:1)Hormone concentration,soaking time,and their interaction exerted highly significant effects(P<0.01)on key growth parameters,including rooting rate,root dry weight,and total root length.Further analysis revealed a nonlinear response of most measured traits to hormone concentration,with the 400 mg/L treatment yielding optimal performance across multiple indicators.Specifically,the C2T1 treatment(400 mg/L for 10 s)resulted in a rooting rate of 92.5%,a root dry weight of 0.035g,and significantly enhanced total root length,number of new shoots,and shoot dry weight compared to other treatments.2)The impact of soaking time was concentration-dependent:extended soaking improved rooting at low concentration(200 mg/L),whereas shorter durations(10 s to 1 min)were more beneficial at medium to high concentrations,and prolonged soaking(60 min)strongly inhibited growth.3)Correlation analysis indicated that rooting rate was significantly positively correlated with root dry weight(P<0.05)and highly significantly correlated with maximum root length,total root length,root number,shoot number,and shoot dry weight(P<0.01).Additionally,root dry weight was also highly significantly correlated with shoot dry weight,suggesting a synergistic relationship between the root system and aboveground growth.4)Principal component analysis(PCA)revealed that C2T1 as the optimal treatment,with its comprehensive score significantly surpassing those of other combinations.In conclusion,this study establishes a “medium concentration-short duration” strategy as highly effective for the cutting propagation of D.odorifera,providing a theoretical insights and practical parameters for large-scale asexual propagation of this valuable species.

Key words: Dalbergia odorifera, cutting propagation, plant growth regulator, rooting rate, shoot growth

中图分类号:

S722.8

杨小丽, 陈飞飞, 黄川腾, 林玲, 陈琳, 黎国运, 董晓娜, 张孟文. 复合生长调节剂(IAA+NAA)对降香黄檀扦插繁殖的调控效应[J]. 林草资源研究, 2025,(5): 53-62.

YANG Xiaoli, CHEN Feifei, HUANG Chuanteng, LIN Ling, CHEN Lin, LI Guoyun, DONG Xiaona, ZHANG Mengwen. Regulatory effects of compound growth regulators (IAA+NAA) on the cutting propagation of Dalbergia odorifera[J]. Forest and Grassland Resources Research, 2025,(5): 53-62.

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指标	浓度			浸泡时间				浓度×浸泡时间
指标	F值	P值		F值		P值		F值		P值
生根率/%	15.33	0.00	10.40		0.00		6.21		0.00
根干质量/g	3.67	0.02	3.29		0.03		4.79		0.00

指标	浓度		浸泡时间		浓度+浸泡时间
指标	F值	P值	F值	P值	F值	P值
总根长/cm	10.12	0.00	4.76	0.00	2.90	0.01
生根数/条	1.12	0.35	14.44	0.00	0.42	0.92
平均根长/cm	1.50	0.23	4.35	0.00	1.41	0.21
最大根长/cm	6.91	0.00	2.83	0.04	1.89	0.08

指标	浓度		浸泡时间		浓度×浸泡时间
指标	F值	P值	F值	P值	F值	P值
新梢数/条	8.13	0.00	3.99	0.01	0.81	0.61
最大新梢长/cm	0.08	0.13	0.11	0.12	0.52	0.14
平均梢长/cm	0.01	0.21	0.36	0.07	0.82	0.09
新梢干质量/g	10.15	0.00	3.29	0.03	2.86	0.00

处理组	第1主成分得分(Y₁)	第2主成分得分(Y₂)	综合得分 (Y)	综合排名
C1T1	-1.15	-1.00	-0.92	12
C1T2	0.34	-0.87	0.13	10
C1T3	0.83	-0.74	0.49	6
C1T4	1.95	-0.30	1.31	3
C2T1	4.27	0.39	3.00	1
C2T2	3.25	-1.24	2.09	2
C2T3	2.00	-1.38	1.21	4
C2T4	0.22	1.24	0.31	9
C3T1	0.94	0.72	0.74	5
C3T2	0.66	-0.62	0.38	8
C3T3	0.56	0.78	0.49	7
C3T4	-1.75	1.11	-1.07	13
C4T1	-0.62	2.36	-0.14	11
C4T2	-2.10	0.18	-1.43	14
C4T3	-2.32	0.78	-1.51	15
C4T4	-7.07	-1.42	-5.06	16