Effects of Drought Stress on Seed Germination and Seedling Growth of Pinus sylvestris var.mongolica from Different Introduction Regions

doi:10.13466/j.cnki.lyzygl.2022.03.010

Abstract

Abstract:

In order to understand the drought resistance of Pinus sylvestris var.mongolica seeds germination and seedling growth,the seeds of P.sylvestris from Zhangwu County,Weichang County,Duolun County,Youyu County,Datong County were collected as study materials,and polyethylene glycol (PEG6000) solution was used to simulate different drought stress treatments.The effects of drought stress on seed germination and seedling growth in these regions were studied and the results showed that the seed germination and seedling growth of P.sylvestris from different introduction regions responded differently to drought stress.The seeds of P.sylvestris from Youyu County adapted to germinate under drought level with a water potential gradient of -0.177 Mpa;seeds from Zhangwu County,Duolun County and Weichang County could germinate under drought level with a water potential gradient of -0.735 Mpa.Except the seeds of P.sylvestris from Datong County,the stronger the drought stress,the slower the germination in other introduction regions.Seedlings of P.sylvestris from Weichang County did not grow well under drought stress.Except Weichang County,the radicle length of seedlings in other introduction regions increased under drought stress compared with CK treatment,and the low drought stress could promote,but high drought stress could inhibit the diameter of seedling embryo axis with the increase of drought stress.According to the analysis,the drought resistance of P.sylvestris seeds in the germination stage from strong to weak was as follows: Datong County>Zhangwu County>Duolun County>Youyu County>Weichang County.Within a certain range,the seeds of P.sylvestris adapted to local environmental conditions,with the drought resistance of the seeds of P.Sylvestris becoming stronger as the region changing to dryer.

Key words: Pinus sylvestris var.Mongolica, seed germination, seedling growth, drought resistance, Three Northern region

CLC Number:

S791.253

ZHANG Risheng, WANG Dongli, WANG Cuiping, CHI Linlin. Effects of Drought Stress on Seed Germination and Seedling Growth of Pinus sylvestris var.mongolica from Different Introduction Regions[J]. FOREST RESOURCES WANAGEMENT, 2022, 0(3): 60-66.

Figures/Tables 7

Tab.1

Fig.1

Fig.2

Fig.3

Tab.2

Tab.3

Tab.4

References 22

[1]	曾德慧, 尤文忠, 范志平, 等. 樟子松人工固沙林天然更新特征[J]. 应用生态学报, 2002, 13(1):1-5.
[2]	Zhu Jiaojun, Fan Zhiping, Zeng Dehui, et al. Comparison of stand structure and growth between artificial and natural forests of Pinus sylvestiris var.mongolica on sandy land[J]. Journal of Forestry Research, 2003, 14(2):103-111. doi: 10.1007/BF02856774
[3]	王德权, 周宇飞, 陆璋镳, 等. 水分胁迫下持绿型高粱根系形态及其活力研究[J]. 干旱地区农业研究, 2012, 30 (2):73-76.
[4]	Miller T E. Effects of emergence time on survival and growth in an early old field plant community[J]. Oecologia, 1987, 72:272-278. doi: 10.1007/BF00379278 pmid: 28311550
[5]	曾德慧, 尤文忠, 范志平, 等. 樟子松人工固沙林天然更新障碍因子分析[J]. 应用生态学报, 2002, 13(3):257-261.
[6]	韩广, 张桂芳, 杨文斌. 影响沙地樟子松天然更新的主要生态气候因子的定量分析[J]. 林业科学, 1999, 35(5):22-27.
[7]	朱教君, 李智辉, 康宏樟, 等. 聚乙二醇模拟水分胁迫对沙地樟子松种子萌发影响研究[J]. 应用生态学报, 2005, 16(5):801-804.
[8]	曾庆平, 郭勇. 植物的逆境应答与系统抗性诱导[J]. 生命的化学(中国生物化学会通讯), 1997, 17(3):31-33.
[9]	郭米山, 王谦, 高广磊, 等. 沙埋和种子处理对樟子松贮藏种子萌发出苗的影响[J]. 中国水土保持科学, 2016, 14(6):86-93.
[10]	姚泽, 王祺, 张元恺, 等. 干旱荒漠区樟子松种子繁育技术研究[J]. 农业与技术, 2013, 33(6):40-41.
[11]	尤国春. 樟子松沙地育苗技术研究[J]. 防护林科技, 2005,(6):17-18.
[12]	李银科, 刘世增, 康才周, 等. 温度对樟子松和沙地云杉种子萌发特征的影响[J]. 水土保持通报, 2011, 31(4):73-76,77.
[13]	高春智, 何炎红, 田有亮, 等. 不同浓度赤霉素浸种对樟子松种子萌发的影响[J]. 内蒙古农业大学学报, 2012, 33(3):67-72.
[14]	王东丽, 张日升, 方祥, 等. 固沙樟子松种子萌发与幼苗生长对干旱胁迫的响应及抗旱性评价[J]. 浙江农林大学学报, 2020, 37(1):60-68.
[15]	国际种子检验协会. 1996国际种子检验规程[M]. 北京: 中国农业出版社, 1999.
[16]	时秀梅. 围场县水资源现状分析与评价[J]. 水利科技与经济, 2013, 19(6):102-105.
[17]	杨鹏晓. 右玉县农村饮水现状调查及分析[J]. 山西水利, 2015(1): 7,9.
[18]	范树宏. 朔州市水面蒸发量特征分析[J]. 山西水利, 2013(7): 10,26.
[19]	马有卿. 大同县水资源现状及可持续利用对策分析[J]. 科技情报开发与经济, 2007, 36(17):262-263.
[20]	阿如旱, 车敏. 近60年多伦县气候特征变化分析[J]. 科技情报开发与经济, 2017, 46(3):426-430.
[21]	武高林, 杜国祯. 植物种子大小与幼苗生长策略研究进展[J]. 应用生态学报, 2008, 19(1):191-197.
[22]	潘昕, 李吉跃, 王军辉, 等. 干旱胁迫对青藏高原4种灌木生理指标的影响[J]. 林业科学研究, 2013, 26(3):352-358.

引种区	采集地	经纬度	海拔 /m	土壤类型	林龄 /a	林分密度/ (株/hm²)	树高 /m	胸径 /cm
ZW	辽宁彰武县章古台镇 (简称彰武县)	42°02'37.914″N 119°35'06.282″E	221	风沙土	28	530	8.09	17.71
WC	河北围场县塞罕坝林场 (简称围场县)	42°25'04.680″N 117°15'58.782″E	1546	风沙土	20	1667	6.12	13.40
DL	内蒙古多伦县大宝生态园 (简称多伦县)	42°08'22.830″N 116°29'20.784″E	1263	风沙土	20	2200	6.50	10.40
YY	山西右玉县南山公园 (简称右玉县)	39°57'53.178″N 112°29'12.606″E	1434	黄绵土	28	1884	8.50	16.00
DT	山西大同县九梁洼林场 (简称大同县)	40°01'55.032″N 113°15'10.782″E	1093	风沙土	27	3210	10.40	15.10

幼苗指标	引种区	CK	T1	T2	T3
鲜重/mg	ZW	39.6±2.13a	27.4±1.41b	30.5±2.35b	8.42±3.78c
	WC	27.3±1.06	—	—	—
	DL	44.8±3.28	37.6±2.34	40.0±2.68	—
	YY	15.4±6.88b	37.6±6.07a	38.3±3.54a	12.92±5.81b
	DT	41.1±5.02a	39.5±4.34ab	28.6±3.33b	31.8±2.20ab
胚芽长/cm	DT	41.1±5.02a	39.5±4.34ab	28.6±3.33b	31.8±2.20ab
	ZW	2.16±0.14ab	1.43±0.06b	1.53±0.07b	0.76±0.08c
	WC	2.27±0.09	—	—	—
	DL	2.18±0.16	2.00±0.10	1.93±0.12	—
	YY	1.73±0.15ab	1.63±0.22ab	1.83±0.14a	1.13±0.03b
	DT	2.10±0.13a	2.33±0.10a	1.45±0.15b	1.56±0.13b
胚根长/cm	DT	2.10±0.13a	2.33±0.10a	1.45±0.15b	1.56±0.13b
	ZW	4.38±0.32ab	3.88±0.13b	5.11±0.58a	3.20±0.14b
	WC	3.28±0.21	—	—	—
	DL	3.20±0.49b	4.95±0.42a	6.06±0.46a	—
	YY	3.57±0.30ab	4.67±0.79ab	6.13±0.47a	2.38±1.10b
	DT	3.07±0.54b	3.13±0.51b	4.65±0.40ab	5.56±0.59a
胚轴直径/mm	DT	3.07±0.54b	3.13±0.51b	4.65±0.40ab	5.56±0.59a
	ZW	0.70±0.02a	0.73±0.02a	0.67±0.02a	0.33±0.15b
	WC	0.67±0.03	—	—	—
	DL	0.78±0.03	0.81±0.02	0.78±0.02	—
	YY	0.35±0.16b	0.62±0.05ab	0.83±0.03a	0.35±0.16b
	DT	0.73±0.03ab	0.85±0.08a	0.8±0.03ab	0.68±0.02b

引种区	萌发率	萌发时滞	鲜重	胚芽长	胚根长	胚轴直径	加权平均值	排序
ZW	0.80	0.15	0.49	0.26	0.66	0.68	0.62	2
WC	0.14	0.60	-0.19	-0.07	-0.20	-0.37	-0.17	5
DL	0.52	0.27	0.55	0.52	0.28	0.49	0.39	3
YY	0.46	0.28	0.41	0.61	0.31	0.37	0.34	4
DT	0.61	0.22	0.70	0.49	0.65	0.83	0.68	1
权重系数	0.051	0.083	0.189	-0.050	0.436	0.290

引种区	海拔/m	降水量/mm	蒸发量/mm	年均温/℃	无霜期/d	干旱指数
辽宁彰武县章古台镇	226.5	491.4	1590.0	6.1	155	3.24
河北围场县塞罕坝机械林场	1500	458.7	979.4	-1.0	64	2.14
山西右玉县	1400	414.4	1018.8	3.5	104	2.46
山西大同县	1157	392.6	1909.0	6.4	125	4.86
内蒙古多伦县	1241	313.2	966.1	1.6	90	3.08