In order to study the most suitable substrate for cutting root-forming of cyclocarya paliurus,the woody branches of the three-year-old cyclocarya paliurus plant were used as the test material,and seven different substracts including perlite,vermiculite,perlite+vermiculite,perlite+biochar-based organic fertilizer,vermiculite+biochar-based organic fertilizer,perlite+peat soil and vermiculite+peat soil were selected for cuttage,the root forming status and the internal Variation characteristics of substances,related enzyme activities and endogenous hormone content were investigated.The results show that,compared with perlite and vermiculite,the addition of biomass biochar-based organic fertilizer can significantly increase the rooting rate,average root length,rooting quantity and rooting index of cuttings of Cyclocarya Paliurus cuttings,and increase SOD,POD,PPO activity and IAA and ABA content,and reduc GA and ZR content,and the addition of biomass biochar-based organic fertilizer and peat soil significantly reduce starch and soluble protein content.In general,perlite+biochar-based organic fertilizer owns highly active enzymey,fully utilized storage materials,hormone which is beneficial to the formation of adventitious roots,and has the best rooting effect,which can be used as a substrate for cuttage of Cyclocarya Paliurus.