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Forest and Grassland Resources Research >

2025 , Vol. 0 >Issue 3: 27 - 37

DOI: https://doi.org/10.13466/j.cnki.lczyyj.2025.03.004

Research paper

Effects of different light intensities on the physiological characteristics of Alsophila spinulosa seedlings

  • Tengmin LI ,
  • Dandan RAO ,
  • Yu CHEN ,
  • Yu HAN ,
  • Mi TIAN
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  • 1. Hainan Academy of Forestry(Hainan Academy of Mangrove),haikou 571100,China
    2. Key Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province,haikou 571100,China

Received date: 2025-04-14

  Revised date: 2025-06-11

  Online published: 2026-01-07

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Abstract

To investigate the effects of different light intensities on physiological characteristics of Alsophila spinulosa seedlings,a pot experiment was conducted under varying shading gradients to control light intensity.Seedlings were exposed to five treatments:Natural greenhouse light intensity(70% transmittance,CK),60% greenhouse light(T1),30% greenhouse light(T2),10% greenhouse light(T3),and 2% greenhouse light(T4).Parameters measured included leaf growth,photosynthetic pigments,antioxidant enzyme activities,membrane stability,and osmotic adjustment substances.The impacts of light intensity were analyzed using correlation analysis and fuzzy comprehensive evaluation.The results showed that:1)The aboveground biomass,underground biomass,and total biomass under T1-T4 treatments were significantly greater than those under CK treatment(P<0.05),while the root-shoot ratio(R/S)was significantly lower than that of CK treatments(P<0.05);2)Under T3 treatment,seedling growth was optimal,with leaf number,leaf length,and leaf width significantly greater than those under other treatments(P<0.05);3)Under T4 treatment,chlorophyll a(Chl a),chlorophyll b(Chl b),and total chlorophyll[Chl(a+b)]were significantly higher than those under other treatment(P<0.05),while carotenoid(Car)content was highest under the T3 treatment;4)As light intensity decreased,the activities of some antioxidant enzyme in the seedlings gradually increased,with superoxide dismutase(SOD)activity reaching its maximum under the T2 treatment;malondialdehyde(MDA)mass fraction,relative electrical conductivity(EC),and soluble sugar(SS)mass fraction gradually decreased,while soluble protein(SP)mass fraction gradually increased;5)Correlation analysis and fuzzy comprehensive evaluation indicated that A.spinulosa seedlings thrived under lower light conditions,with T3 treatment(10% greenhouse light intensity)identified as the optimal growth environment for them.These findings provide quantifiable key parameters for the population restoration of A.spinulosa,a Nationally Key Protected Wild Plant(Class II)in China-thereby enhancing its conservation success.

Key words： Alsophila spinulosa; light intensity; photosynthetic pigment; physiologic index; seedlings

Cite this article

Tengmin LI , Dandan RAO , Yu CHEN , Yu HAN , Mi TIAN . Effects of different light intensities on the physiological characteristics of Alsophila spinulosa seedlings[J]. Forest and Grassland Resources Research, 2025 , 0(3) : 27 -37 . DOI: 10.13466/j.cnki.lczyyj.2025.03.004

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