为研究青钱柳扦插生根的最适基质,以3a生青钱柳母株上1a木质化枝条为试验材料,选用珍珠岩、蛭石、珍珠岩+蛭石、珍珠岩+生物质炭基有机肥、蛭石+生物质炭基有机肥、珍珠岩+泥炭土和蛭石+泥炭土7种不同扦插基质,研究在不同基质处理下,青钱柳扦插过程中的生根状况、内含物质、相关酶活性和内源激素含量的变化特征。结果表明:与珍珠岩和蛭石相比,添加生物质炭基有机肥处理能够显著增加青钱柳插穗的生根率、平均根长、生根数量和生根指数,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)的活性、生长素(IAA)和脱落酸(ABA)含量,降低赤霉素(GA)和玉米素核苷(ZR)的含量;添加生物质炭基有机肥和泥炭土均显著降低了淀粉和可溶性蛋白含量。总体来看,珍珠岩+生物质炭基有机肥处理的酶活性较高、储藏物质能够充分利用,激素含量更有利于不定根的形成,生根效果最好,可作为青钱柳扦插繁殖的基质。

In order to study the most suitable substrate for cutting root-forming of cyclocarya paliurus,the woody branches of the three-year-old cyclocarya paliurus plant were used as the test material,and seven different substracts including perlite,vermiculite,perlite+vermiculite,perlite+biochar-based organic fertilizer,vermiculite+biochar-based organic fertilizer,perlite+peat soil and vermiculite+peat soil were selected for cuttage,the root forming status and the internal Variation characteristics of substances,related enzyme activities and endogenous hormone content were investigated.The results show that,compared with perlite and vermiculite,the addition of biomass biochar-based organic fertilizer can significantly increase the rooting rate,average root length,rooting quantity and rooting index of cuttings of Cyclocarya Paliurus cuttings,and increase SOD,POD,PPO activity and IAA and ABA content,and reduc GA and ZR content,and the addition of biomass biochar-based organic fertilizer and peat soil significantly reduce starch and soluble protein content.In general,perlite+biochar-based organic fertilizer owns highly active enzymey,fully utilized storage materials,hormone which is beneficial to the formation of adventitious roots,and has the best rooting effect,which can be used as a substrate for cuttage of Cyclocarya Paliurus.