为建立优良银白杨离体快繁再生体系,培育速生高产的银白杨苗木,以西藏银白杨叶片、茎段作为不定芽诱导的初始材料,以MS为基本培养基,对不定芽诱导中产生的愈伤组织进行二次不定芽诱导,研究不同激素浓度及配比对银白杨不同材料不定芽诱导与增殖分化的影响。研究表明:6-BA∶NAA为5∶1是银白杨不定芽诱导的最优激素浓度配比,银白杨不定芽诱导的最佳培养材料为当年生带腋芽茎段;6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L)为愈伤组织诱导不定芽最优激素浓度组合;不定芽增殖的最优激素7浓度组合为6-BA(0.5mg/L)+NAA(0.2mg/L)+KT(0.2mg/L)+IBA(0.2mg/L),平均增殖系数最高。综合考虑得出:MS+6-BA(0.5mg/L)+NAA(0.1mg/L)为银白杨不定芽诱导分化的最佳培养基,不定芽诱导的最佳材料为银白杨当年生带腋芽茎段;愈伤组织不定芽诱导的最佳培养基为MS+6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L);不定芽增殖的最佳培养基为MS+6-BA(1.0mg/L)+NAA(0.2mg/L)+KT(0.2mg/L)+IBA(0.2mg/L),pH5.8。

To establish a rapid regeneration system of Populusalba in vitro,and to cultivate fast-growing and high-yielding silver poplar seedlings.The leaves and stem segments of Populusalba were used as the initial material for adventitious bud induction,and the adventitious bud induction was induced in the callus induced by adventitious bud induction.Using MS as the basic medium,the effects of different hormone concentrations and ratios on the induction of adventitious buds of different materials of Populusalba were studied.The results showed that 6-BA:NAA was 5:1,which was the optimal ratio of hormone concentration induced by adventitious buds of Populusalba. The best culture material for adventitious bud induction of Populusalba was the same year.6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L)is the optimal hormone concentration combination for callus induction of adventitious buds.The optimal hormone concentration combination for adventitious bud proliferation was 6-BA(0.5 mg/L)+NAA(0.2 mg/L)+KT(0.2 mg/L)+IBA(0.2 mg/L),and the average proliferation coefficient was 8.3.MS+6-BA(0.5mg/L)+NAA(0.1mg/L)is the best medium for adventitious bud induction of Populus alba. The best material for adventitious bud induction is the white bud stem stage.The optimal medium for callus induction of callus was MS+6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L);the best medium for adventitious bud proliferation was MS+6-BA(1.0 mg/L)+NAA(0.2 mg/L)+KT(0.2 mg/L)+IBA(0.2 mg/L),pH 5.8.