探讨长圆叶黄脉爵床对生物和非生物胁迫的生理响应,可为园林植物抗逆性育种和有害生物管理提供科学依据。以受剪叶和褐云玛瑙螺取食胁迫后的长圆叶黄脉爵床叶片为研究对象,测定剪叶与褐云玛瑙螺取食胁迫后不同时长条件下叶片的叶绿素与茶多酚含量及苯丙氨酸解氨酶活性。结果表明:1)褐云玛瑙螺喜食长圆叶黄脉爵床叶片,其取食量较大,常导致叶片被取食殆尽,严重影响长圆叶黄脉爵床的光合作用;2)剪叶及褐云玛瑙螺取食胁迫对长圆叶黄脉爵床叶片的叶绿素与茶多酚含量及苯丙氨酸解氨酶活性均存在显著影响;3)剪叶与褐云玛瑙螺取食胁迫后3、9、15 h时长下均能诱导长圆叶黄脉爵床叶片合成更多的叶绿素和茶多酚,但苯丙氨酸解氨酶活性迅速降低,显著低于对照组,表明苯丙氨酸被积累有利于修复伤口;4)褐云玛瑙螺取食生物胁迫对长圆叶黄脉爵床叶片叶绿素与茶多酚含量以及苯丙氨酸解氨酶活性的诱导反应比剪叶非生物胁迫更迅速。长圆叶黄脉爵床受剪叶与褐云玛瑙螺取食胁迫的生理响应表现为叶绿素与茶多酚含量增加,苯丙氨酸解氨酶活性被抑制。

Exploring the physiological response of Sanchezia oblonga to biotic and abiotic stress,in order to provide a scientific basis for carrying out stress resistance breeding or pest management of garden plants.The study focused on the leaves of S.oblonga under the biotic stress feeding of Achatina fulica and the abiotic stress of leaf cutting.Chlorophyll and tea polyphenol content as well as phenylalanine ammonia lyase activity of the leaves were measured under different growth conditions after leaf cutting and snail feeding stress.The results showed that A.fulica prefer to feed on the leaves of S.oblonga,and their feeding amount is large,which often leads to the leaves being completely consumed,seriously affecting the photosynthesis of S.oblonga.The leaf cutting stimulation and snail feeding stress have significant effects on the chlorophyll content,tea polyphenol content,and phenylalanine ammonia lyase(PAL)activity of the leaves of S.oblonga.At 3,9 and 15 hours after snail feeding and leaf cutting stimulation stress can induce more chlorophyll and tea polyphenols to be synthesized in the leaves of S.oblonga.However,PAL activity decreased rapidly and was significantly lower than that of the control group,suggesting that phenylalanine was accumulating to promote wound repair.The effects of snail feeding on chlorophyll,tea polyphenol content and PAL activity were more rapid than those induced by leaf cutting abiotic stress.In conclusion,the physiological response of S.oblonga after leaf cutting stimulation and snail feeding stress showed that the content of chlorophyll and tea polyphenols increased,and the activity of PAL was inhibited.