A Study on Community Characteristics of Forest Soil Bacteria in Lushan National Nature Reserve

doi:10.13466/j.cnki.lyzygl.2019.06.018

Abstract

Abstract:

Soil microbe is ecologically important contributor to various functioning of forest ecosystems.Because of the elevation gradient,subtropical mountainous forests are a complex ecosystem where the nutrient availability and soil conditions vary with elevation levels.Therefore,the subtropical mountainous forests provide aunique opportunity to study the relationship between forest microbial communities and multiple environmental factors.In this study,we present a comprehensive analysis of soil bacterial community composition and diversity along six elevations representing six typical vegetation types in a subtropical mountainous forest at the Lushan,China.A total of 24 phyla,49 classes and 62 orders were confirmed with the method of high-throughput sequencing technology.At the phylum level,the dominant phyla were Acidobacteria(53.84%),Proteobacteria(30.15%)and Actinobacteria(7.36%);other phyla,such as Bacteroidetes and Chloroflexi(0.64% and 0.28%),were much less abundant.Ellin 6513 was the most abundant order in soil bacterial community.The composition and diversity of bacterial communities did not show a certain trend across an elevation gradient from low via medium to high.Among the soil chemical indexes,the organic carbon and total mitrogen confent are more relevent to the soil bacterial community in the Lushan forest.

Key words: soil microbe, high-throughput sequencing, bacterial community structure, elevation, Lushan

CLC Number:

FENG Xiaochuan, CAO Xinguang, JU Wenhua, LIU kaimei. A Study on Community Characteristics of Forest Soil Bacteria in Lushan National Nature Reserve[J]. FOREST RESOURCES WANAGEMENT, 2019, 0(6): 101-107.

Figures/Tables 6

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样品编号	取样位置	经纬度	海拔/m	土壤类型	森林植被类型	主要层优势植物
HHP-H1	好汉坡	29°35'57″N,115°59'15″E	570	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HHP-H2	好汉坡	29°35'45″N,115°39'11″E	680	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HHP-H3	好汉坡	29°35'33″N,115°59'09″E	776	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HLS-H4	黄龙寺	29°33'06″N,115°57'50″E	975	山地黄棕壤	常绿与落叶阔叶混交林	毛竹、小叶白辛树、樟树
DYS-H5	大月山	29°33'53″N,115°59'04″E	1250	山地棕壤	针阔混交林	短柄枹、花柏;微毛柃
WLF-H6	五老峰	29°32'55″N,116°00'49″E	1350	山地棕壤	针叶林	黄山松、杜娟

样品编号	枯枝落叶层厚度/cm	土壤总氮/(g/kg)	有机碳/(g/kg)	pH
HHP-H1	8	3.22	42.83	4.28
HHP-H2	6	4.72	68.55	4.26
HHP-H3	5	5.29	74.75	4.25
HLS-H4	4	5.43	78.29	4.54
DYS-H5	6	4.54	70.76	4.20
WLF-H6	3	4.78	73.21	3.88

样品编号	优化序列	OTUs数/个	Chao 1指数	Simpson指数	Shannon指数
HHP-H1	23031	342	375.454	0.972	6.266
HHP-H2	25314	343	352.282	0.980	6.520
HHP-H3	22071	346	355.692	0.973	6.331
HLS-H4	22947	366	382.912	0.985	6.856
DYS-H5	21144	325	340.857	0.976	6.433
WLF-H6	22206	360	384.972	0.981	6.630