Abstract:

To establish a rapid regeneration system of Populusalba in vitro,and to cultivate fast-growing and high-yielding silver poplar seedlings.The leaves and stem segments of Populusalba were used as the initial material for adventitious bud induction,and the adventitious bud induction was induced in the callus induced by adventitious bud induction.Using MS as the basic medium,the effects of different hormone concentrations and ratios on the induction of adventitious buds of different materials of Populusalba were studied.The results showed that 6-BA:NAA was 5:1,which was the optimal ratio of hormone concentration induced by adventitious buds of Populusalba. The best culture material for adventitious bud induction of Populusalba was the same year.6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L)is the optimal hormone concentration combination for callus induction of adventitious buds.The optimal hormone concentration combination for adventitious bud proliferation was 6-BA(0.5 mg/L)+NAA(0.2 mg/L)+KT(0.2 mg/L)+IBA(0.2 mg/L),and the average proliferation coefficient was 8.3.MS+6-BA(0.5mg/L)+NAA(0.1mg/L)is the best medium for adventitious bud induction of Populus alba. The best material for adventitious bud induction is the white bud stem stage.The optimal medium for callus induction of callus was MS+6-BA(0.5mg/L)+NAA(0.1mg/L)+KT(0.2mg/L);the best medium for adventitious bud proliferation was MS+6-BA(1.0 mg/L)+NAA(0.2 mg/L)+KT(0.2 mg/L)+IBA(0.2 mg/L),pH 5.8.

Key words: Tibet, Populus alba, adventitious bud induction, adventitious bud proliferation