Effects of Different Light Intensity on Physiological Characteristics of One-Year-Old Bruguiera gymnorhiza Seedlings

doi:10.13466/j.cnki.lyzygl.2023.04.006

Abstract

Abstract:

This study was conducted to investigate the light adaptability of 1-year-old Bruguiera gymnorhiza(L.)Lam.seedlings.The seedlings of Bruguiera gymnorrhiza were treated by light intensity simulation control experiment.The indices of plant height,basal diameter,Chl(a+b)content,Pn,MDA content,PRO content and SOD activity were measured after 20%,40%,60%,80% and 100% light intensity treatments.The effects of light on Bruguiera gymnorrhiza seedlings were analyzed by correlation and principal component analysis.The results showed,1)60% light intensity environment of Bruguiera gymnorrhiza seedling height growth fastest,100% light intensity environment of Bruguiera gymnorrhiza seedling base diameter growth fastest.2)The total chlorophyll content decreased with the increase of light intensity,but the net photosynthetic rate increased first and then decreased with the increase of light intensity.There was no significant difference in total chlorophyll content and net photosynthetic rate between 60% light intensity and 80% and 100% light intensity.3)Under 60% light intensity environment,the malondialdehyde content and proline content of Bruguiera gymnorrhiza were significantly lower than those under 20% light intensity environment,but the superoxide dismutase activity was significantly higher than that under 20% light intensity environment.4)There was no significant difference in net photosynthetic rate,malondialdehyde content and proline content between 60% light intensity and 100% light intensity,but the activity of superoxide dismutase was significantly higher than that of 100% light intensity.5)Through correlation and principal component analysis,it was found that 60% light intensity environment was the best for the growth of Bruguiera gymnorrhiza seedlings.Therefore,the adaptability of 1-year-old seedlings to 60% light intensity was stronger than that of other light environments.The results showed that 100% light intensity had a certain light inhibition effect on 1 a Bruguiera gymnorrhiza seedlings,while 20% and 40% light intensity could not meet the needs of 1 a Bruguiera gymnorrhiza seedlings for light.The results provide a numerical reference for the appropriate shading treatment of Bruguiera gymnorrhiza seedlings in the process of ecological restoration.

Key words: Bruguiera gymnorhiza, light intensity, morphological indicators, physiological indicators, principal component analysis

CLC Number:

Q945.3

FANG Fazhi, LI Zhaojia, ZHANG Mengwen, WU Zhongqin, GUI Huiying. Effects of Different Light Intensity on Physiological Characteristics of One-Year-Old Bruguiera gymnorhiza Seedlings[J]. FOREST RESOURCES WANAGEMENT, 2023, 0(4): 43-52.

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References 30

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光照强度/%	初始株高/cm	最终株高/cm	株高增长量/cm	株高增长率/%
20	20.11±1.72a	48.53±3.30b	28.42±2.84b	143.7±0.21b
40	20.95±0.90a	55.18±2.10ab	34.22±2.84b	165.01±0.20ab
60	20.16±0.17a	62.83±1.51a	42.66±1.45a	211.58±0.07a
80	20.96±1.25a	53.40±2.50a	32.44±2.63b	154.77±0.25b
100	22.73±1.21a	57.18±2.47a	34.45±1.76b	152.13±0.09b

光照强度/%	初始基径/mm	最终基径/mm	基径增长量/mm	基径增长率/%
20	6.55±0.34a	19.13±1.72b	12.58±1.52b	191.78±0.21b
40	7.30±0.28a	26.72±3.41b	19.42±3.21b	264.36±0.38b
60	7.15±0.54a	28.42±3.76b	21.27±3.76b	300.92±0.56b
80	6.85±0.36a	40.86±3.24 a	34.01±4.55a	496.50±2.20a
100	6.15±0.56a	45.37±6.23a	39.22±6.07a	642.86±1.03a

光照强度/%	Chl(a)/(mg/g)	Chl(b)/(mg/g)	Chl总含量/(mg/g)
20	0.270 0±0.026 4a	0.353 3±0.056 1a	0.623 3±0.081 1a
40	0.260 0±0.005 8ab	0.280 0±0.045 8a	0.540 0±0.051 3ab
60	0.163 3±0.006 7c	0.236 7±0.038 4a	0.400 0±0.043 6ab
80	0.232 0±0.006 9b	0.255 5±0.021 4a	0.465 4±0.013 3b
100	0.220 0±0.005 8b	0.256 7±0.020 3a	0.473 3±0.014 53b

生长指标	株高增长量	基径增长量	株高增长率	基径增长率	Pn	Chl 总含量	MDA 含量	SOD 活性	PRO 含量
株高增长量	1.000
基径增长量	0.184	1.000
株高增长率	0.903^**	-0.006	1.000
基径增长率	0.183	0.971^**	-0.044	1.000
Pn	0.705^*	0.563	0.522	0.541	1.000
Chl总含量	-0.697^*	-0.337	-0.453	-0.303	-0.692^*	1.000
MDA含量	-0.620^*	-0.470	-0.419	-0.404	-0.661^*	0.714^**	1.000
SOD活性	0.848^**	0.123	0.833^**	0.042	0.704^*	-0.649^*	-0.596^*	1.000
PRO含量	-0.635^*	-0.644^*	-0.344	-0.631^*	-0.844^**	0.742^**	0.699^*	-0.639^*	1.000

成份	初始特征值			提取平方和载入
成份	特征值	方差贡献率/%	累积贡献率/%	特征值	方差贡献率/%	累积贡献率/%
1	4.432	49.24	49.24	4.432	49.24	49.24
2	3.263	36.26	85.50	3.263	36.26	85.50
3	1.213	13.48	98.98	1.213	13.48	98.98
4	0.092	1.02	100.00