Phenotypic Variation and Early Selection of Half-sib Families of Pinus yunnanensis

doi:10.13466/j.cnki.lczyyj.2024.01.014

Abstract

Abstract:

Pinus yunnanensis is a unique native species to Southwest China,distributed in the southeastern margin of the Qinghai-Tibet Plateau,and its genetic degradation is a serious concern.To accelerate the breeding process and diversify the genetic resources,95 half-sib families of P.yunnanensis were evaluated and selected in the early stage based on the systematic analysis of nutritional growth traits(plant height,ground diameter,long crown diameter,short crown diameter,height under the branches,length of primary branches in the current year,and number of lateral branches in the current year) and reproductive growth traits(number of cones).The results indicated that:1)there were significant differences in nutritional growth traits among families of P.yunnanensis,indicating that it had rich genetic variation and selection potential.2)The coefficient of variation and heritability of phenotypic traits were 21.60%~86.07%and 0.75~0.78,respectively,indicating that the traits of P.yunnanensis had large variation,and they are strongly controlled by genetics.3)There is a strong correlation between phenotypic traits.By performing cluster analysis on the nutritional growth traits,the 95 families can be divided into three categories.4)By conducting a membership function analysis on the nutritional growth traits of P.yunnanensis,10 superior families were selected,and the genetic gain and actual gain of plant height were up to 19.63% and 25.17%,respectively,and ground diameter were up to 12.39% and 15.89%,respectively.There were abundant phenotypic variations among half-sib families of P.yunnanensis,and 10 superior families were selected based on vegetative growth traits.The results can provide material support and a theoretical basis for the breeding of improved varieties of P.yunnanensis.

Key words: Pinus yunnanensis, half-sib families, genetic variation, early selection

CLC Number:

S722

LI Zhongmu, CHE Fengxian, GAO Chengjie, LI Jin, WANG Lu, CUI Kai. Phenotypic Variation and Early Selection of Half-sib Families of Pinus yunnanensis[J]. Forest and Grassland Resources Research, 2024, (1): 102-110.

Figures/Tables 9

Tab.1

The formula of data processing

公式名称	公式	参数说明	参考文献
混合线性模型	Y_ijk=μ+B_i+F_j+BF_ij+e_ijk	Y_ijk为第i区组第j家系第k单株的观测值;μ为群体平均值;B_i为第i区组效应;F_j为第j家系的效应;BF_ij为第i家系和第j区组的互作效应;e_ijk为随机误差。此外,μ和B_i为固定效应,其余为随机效应。	[26]
遗传力	$h F 2$ = $σ F 2$ /( $σ E 2 n b$ + $σ F B 2 b$ + $σ F 2$ )	n为小区单株数的调和值;b为区组数; $σ F 2$ 为家系方差分量; $σ E 2$ 为随机误差; $σ F B 2$ 为家系与区组相互作用的方差分量。	[27]
表型变异系数	V_CVP= $σ p 2$ / $X —$ ×100%	$σ p 2$ 为表型方差分量; $X —$ 为表型性状的群体均值。	[28]
表型相关性	r_p=Covp₁₂/ $σ p 1 2 × σ p 2 2$	Covp₁₂为表型性状的协方差; $σ p 1 2$ 和 $σ p 2 2$ 分别两个性状的表型方差。	[27]
隶属函数	μ_A=(X_i-X_min)/(X_max-X_min)	X_i为表型性状的测量值;X_min为表型性状的最小值;X_max为表型性状的最大值。
遗传增益	G=(X_i- $X —$ )/ $X —$ × $h F 2$ ×100%	$X -$ 为所选家系性状的平均值。	[26]
实际增益	ΔG=(X_i- $X —$ )/ $X —$ ×100%	$X -$ 为所选家系性状的平均值。	[26]

Tab.1

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Fig.1

Fig.2

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Fig.4

Tab.4

Tab.5

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性状	F			方差分量百分比%
性状	家系	区组	家系×区组	家系	区组	家系×区组
树高	3.784^**	28.536^**	3.393^**	10.60	79.90	9.50
地径	3.896^**	45.086^**	3.455^**	7.43	85.98	6.59
长冠径	3.819^**	28.295^**	3.299^**	10.79	79.90	9.32
短冠径	3.807^**	52.492^**	3.209^**	6.40	88.21	5.39
枝下高	2.310^**	7.490^**	1.888^**	19.76	64.08	16.16
当年生侧枝数	2.999^**	5.932^**	2.771^**	25.63	50.69	23.68
当年生主枝长	3.897^**	26.112^**	3.657^**	11.58	77.56	10.86
球果数量	1.129	0.235	0.520	59.91	12.50	27.60

性状	变化范围	均值	标准差	表型变异系数/%	遗传力
树高/cm	106.55~203.13	164.43	45.98	27.96	0.78
地径/mm	41.90~ 67.17	58.34	12.60	21.60	0.78
长冠径/cm	76.58~159.53	128.07	36.90	28.81	0.78
短冠径/cm	66.18~148.60	111.51	35.30	31.66	0.78
枝下高/cm	23.47~ 48.90	36.13	13.37	37.01	0.76
当年生侧枝数	2.82~ 7.39	3.60	1.38	38.33	0.76
当年生主枝长/cm	37.17~ 83.00	58.59	20.48	34.95	0.77
球果数量	0~ 28.00	6.10	5.25	86.07	0.75

家系	树高	地径	长冠径	短冠径	枝下高	当年生侧枝数	当年生主枝长	球果数量
11	25.17	10.87	19.61	27.62	-34.93	3.66	38.88	-37.26
18	23.56	15.89	20.37	22.68	-13.12	16.21	32.55	-10.37
25	9.77	14.61	10.97	14.76	-14.04	-1.43	19.12	-100.00
27	22.27	15.15	26.09	35.08	-6.04	16.29	20.99	34.44
44	18.49	12.34	15.33	14.84	7.94	-0.51	22.54	79.26
50	13.91	2.48	15.31	16.32	16.40	3.57	16.19	-19.34
56	15.65	12.71	19.01	21.40	14.36	6.43	19.91	7.55
68	15.33	14.15	17.48	21.98	6.38	3.66	21.84	-37.26
83	10.94	13.64	14.44	17.84	21.22	0.05	2.65	-10.37
96	12.08	5.89	19.74	19.39	7.32	9.01	12.68	85.98

家系	树高	地径	长冠径	短冠径	枝下高	当年生侧枝数	当年生主枝长	球果数量
11	19.63	8.48	15.30	21.55	-26.55	2.78	29.94	-27.95
18	18.37	12.39	15.89	17.69	-9.97	12.32	25.06	-7.78
25	7.62	11.39	8.56	11.51	-10.67	-1.08	14.72	-75.00
27	17.37	11.82	20.35	27.36	-4.59	12.38	16.16	25.83
44	14.43	9.62	11.96	11.57	6.03	-0.39	17.36	59.44
50	10.85	1.93	11.94	12.73	12.46	2.71	12.47	-14.50
56	12.20	9.91	14.83	16.70	10.91	4.89	15.33	5.66
68	11.96	11.04	13.63	17.14	4.85	2.78	16.82	-27.95
83	8.53	10.64	11.27	13.92	16.13	0.03	2.04	-7.78
96	9.42	4.60	15.39	15.13	5.56	6.85	9.76	64.48